TOF may refer to:

• The Original Factory Shop, a British discount department store
• Time of flight, a principle of several scientific methods
  • Time-of-flight camera, a camera system that analyzes time of flight of the light it captures, to resolve depth
  • Time-of-flight mass spectrometry uses the time of flight of an ion in the gas phase to resolve its mass-to-charge ratio
• Tetralogy of Fallot, a congenital heart defect
• TOF, British abbreviation for Tracheoesophageal fistula, an abnormal connection between the esophagus and the trachea
• Turnover frequency, a rate in chemical reactions
• TOF, IATA airport code for Bogashevo Airport, in Tomsk, Russia
• Torch of Freedom, a novel by David Weber
• TOF, MTR station code for Tong Fong Tsuen stop, in Hong Kong
• TOF (Top of Form), relates to the print start position i.e. the position the printer starts printing from at the top of the page
• Tower of Fantasy, a video game

Tof may refer to:

• Tof, alternate term for the Tofalar people of Siberian Russia
• The timbrel or hand-drum in the Hebrew music of Old Testament times
• Tof, green-skinned humanoids from planet Tof, from the Star Wars universe